beta Amyloid anticorps (N-Term)

N° du produit ABIN401205

Cet anticorps anti-beta Amyloid est un anticorps Lapin Polyclonal détectant beta Amyloid dans WB, IF, EIA et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour beta Amyloid anticorps (N-Term) (ABIN401205)

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Amyloid est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-beta Amyloid anticorps

Épitope: AA 1-14, N-Term

Séquence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography

Immunogène: Synthetic peptide corresponding to the amino terminus (1-14) of Human beta amyloid conjugated to KLH using maleimide.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/10,000. Western blot: 0.5-1.0 μg/mL. A 40-50 kD band consistent with a higher MW precursor isdetected in using whole tissue extracts from mouse brain. Immunofluorescence. Immunohistochemistry: We recommend the use of 4 % PFA for Paraffin embedded tissuesand 10 % Formalin for Frozen tissue for fixation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at-20 °C or below for longer .

Détails sur beta Amyloid

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Autre désignation: Amyloid beta

Sujet: Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.Synonyms: Amyloid beta peptide

Pathways: Inflammasome